Progression of osteogenic cell cultures grown on microtopographic titanium coated with calcium phosphate and functionalized with a type I collagen-derived peptide.

BACKGROUND: The functionalization of metallic surfaces aims at promoting the cellular response at the biomaterial-tissue interface. This study investigates the effects of the functionalization of titanium (Ti) microtopography with a calcium phosphate (CaP) coating with and without peptide 15 (P-15), a synthetic peptide analog of the cell-binding domain of collagen I, on the in vitro progression of osteogenic cells. METHODS: Sandblasting and acid etching (SBAE; control) Ti microtopography was coated with CaP, enabling the loading of two concentrations of P-15: 20 or 200 µg/mL. A machined Ti was also examined. Rat calvarial osteogenic cells were cultured on Ti disks with the surfaces mentioned above for periods up to 21 days (n = 180 per group). RESULTS: CaP coating exhibited a submicron-scale needle-shaped structure. Although all surfaces were hydrophobic at time zero, functionalization increased hydrophilicity at equilibrium. Microtopographies exhibited a lower proportion of well-spread cells at 4 hours of culture and cells with long cytoplasmic extensions at day 3; modified SBAE supported higher cell viability and larger extracellular osteopontin (OPN) accumulation. For SBAE and modified SBAE, real-time polymerase chain reaction showed the following results: 1) lower levels for runt-related transcription factor 2 at 7 days and for bone sialoprotein at days 7 and 10 as well as higher OPN levels at days 7 and 10 compared to machined Ti; and 2) higher alkaline phosphatase levels at day 10 compared to day 7. At 14 and 21 days, modified SBAE supported higher proportions of red-dye-stained areas (calcium content). CONCLUSION: Addition of a CaP coating to SBAE Ti by itself may affect key events of in vitro osteogenesis, ultimately resulting in enhanced matrix mineralization; additional P-15 functionalization has only limited synergistic effects.

Effect of chemical denture cleansers on flexural resistance and color changes of microwave-polymerized acrylic resins

Aim: The aim of this study was to assess the flexural resistance and color alterations of microwave-polymerized acrylic resins immersed in denture cleansers for different periods of time. Methods: Forty-five rectangular specimens (65x10x3mm) of each commercial brand of the microwave-activated acrylic resins (Vipi Wave and Onda Cryl) were divided in three denture cleanser groups (Bony Plus, Corega Tabs and Efferdent Plus) and a control group (immersion in water). Soaking trials of 15 min and 8 h simulated 30 days of use. The flexural strength test was carried out in a universal testing machine. Color alterations were assessed by visual inspection of photographs. The results obtained in the flexural test, in kgf, were converted to MPa and these values were submitted to analysis of variance with a 5% significance level. Results: There were no significant differences (p<0.05) between Onda Cryl (85.61±12.76) and Vipi Wave (89.8±19.95) after the soaking trials regarding the use of different denture cleansers. No differences were found in relation to the solutions [Bony Plus (88.52±9.89), Corega Tabs (88.75±12.71) and Efferdent (85.86±12.11)], soaking periods [control (87.17±12.92), 15 min (88.05±11.74) and 8 h (87.91±10.30)], and interactions during the 30 days of simulated use. Visual inspection did not detect any color alterations. Conclusions: Denture cleansers, when used according to the manufacturers’ instructions, did not cause any mechanical or visual alterations in the microwave-polymerized acrylic resins after a simulated period of 30 days of use.

Assessment of flexural strength and color alteration of heat-polymerized acrylic resins after simulated use of denture cleansers

O objetivo deste estudo foi avaliar a resistência à flexão e alterações de cor de resinas acrílicas termopolimerizáveis imersas em higienizadores de prótese por diferentes períodos de tempo. Corpos-de-prova retangulares (65x10x3mm) de três resinas acrílicas (Lucitone 550, QC-20 e Triplex) foram divididos em três grupos de higienizadores de prótese (Bony Plus, Corega Tabs e Efferdent Plus) e um grupo controle (imersão em água). Foram realizadas imersões por 15 min e 8 horas, simulando 30 dias de uso. O teste de resistência à flexão foi realizado com 105 corpos-de-prova em máquina de ensaios universais. Alterações de cor foram avaliadas por meio de fotografias tiradas de 21 corpos-de-prova. Os resultados de resistência à flexão (em MPa) foram submetidos à análise de variância e teste de Tukey, com nível de significância de 5%. Houve diferenças significantes (p<0.01) entre as resinas Lucitone (89,439 ± 7,962); Triplex (88,024 ± 5,167) and QC-20 (83,379 ± 7,153). Não foram encontradas diferenças significantes (p>0.05) entre os higienizadores de prótese (Bony Plus = 87,693 ± 6,943; Corega Tabs = 86,955 ± 7,114; Efferdent Plus = 86,195 ± 7,865; e controle = 86,536 ± 7.012) nem entre os períodos de imersão (15 min = 86,875 ± 7,625 e 8 h = 87,432 ± 7,355) durante os ciclos de imersão que simularam 30 dias de uso. A inspeção visual não detectou qualquer alteração de cor. Os achados desse estudo revelaram que os higienizadores de prótese, quando usados de acordo com as instruções dos fabricantes, não afetaram a resistência à flexão nem causaram alterações de cor nas resinas acrílicas termopolimerizáveis após um período de uso simulado de 30 dias.

Assessment of flexural strength and color alteration of heat-polymerized acrylic resins after simulated use of denture cleansers.

The purpose of this study was to assess flexural strength and color alteration of acrylic resins immersed in denture cleansers for different periods of time. Rectangular specimens (65 x 10 x 3mm) made from three heat-polymerized acrylic resins (Lucitone 550, QC-20 and Triplex) were assigned to three denture cleansers groups (Bony Plus, Corega Tabs and Efferdent Plus) and a control group (immersion in water). Soaking trials of 15 min and 8 h simulated 30 days of use. Flexural strength testing was carried out with 105 specimens on a universal testing machine. Color alterations were visually assessed by examination of photographs taken from 21 specimens. Flexural strength means (in MPa) were analyzed statistically by analysis of variance and Tukey's test at 5% significance level. There were significant differences (p<0.01) among the resins Lucitone (89.439 +/- 7.962), Triplex (88.024 +/- 5.167) and QC-20 (83.379 +/- 7.153). No significant differences (p>0.05) were found either among the denture cleansers (Bony Plus = 87.693 +/- 6.943; Corega Tabs = 86.955 +/- 7.114; Efferdent Plus = 86.195 +/- 7.865 and control = 86.536 +/- 7.012) or between the soaking periods (15 min = 86.875 +/- 7.625 and 8 h = 87.432 +/- 7.355) throughout the soaking cycles simulating 30 days of use. No color alterations were identified by visual examination. The findings of this study showed that chemical denture cleansers used according to the manufacturers' specifications did not cause flexural strength alterations or color changes in heat-polymerized acrylic resins submitted to soaking cycles that simulated 30 days of use.

Evaluation of transfer impressions for osseointegrated implants at various angulations.

The accuracy of impressions that transfer the relationship of the implant to the metal framework of the prosthesis continues to be a problem. This study was designed to evaluate the accuracy of the transfer process under variable conditions with regard to implant analog angulations, impression materials, and techniques. Replicas (n = 60) of a metal matrix (control) containing four implants at 90 degrees , 80 degrees , 75 degrees , and 65 degrees in relation to the horizontal surface were obtained by using three impression techniques: T1-indirect technique with conical copings in closed trays; T2-direct technique with square copings in open trays; and T3-square copings splinted with autopolymerizing acrylic resin; and four elastomers: "P"-polysulfide; "I"-polyether; "A"-addition silicone; and "Z"-condensation silicone. The values of the implant analog angulations were assessed by a profilometer to the nearest 0.017 degrees , then submitted to analysis of variance for comparisons at significance of 5% (P < .05). For implant analog at 90 degrees , the material "A" associated with T2 and material "Z" with T3 behaved differently (P < .05) from all groups. At 80 degrees , all materials behaved differently (P < .01) with T1. At 75 degrees , when T1 was associated, materials "P" and "A" showed similar behavior, as well as materials "I" and "Z"; however, "P" and "A" were different from "I" and "Z" (P < .01). When T3 was associated, all experimental groups behaved differently among them (P < .01). At 65 degrees , the materials "P" and "Z" behaved differently (P < .01) from the control group with T1, T2, and T3; the materials "I" and "A" behaved differently from the control group (P < .01) when T1 and T2, respectively, were associated. The more perpendicular the implant analog angulation is in relation to the horizontal surface, the more accurate the impression. The best materials were material "I" and "A" and the most satisfactory technique was technique 3.

Effect of casting technique on surface roughness and consequent mass loss after polishing of NiCr and CoCr base metal alloys: a comparative study with titanium.

STATEMENT OF PROBLEM: Surface roughness of cast metal frameworks may lead to difficulties in finishing or polishing procedures and weaken the framework. PURPOSE: The aim of this study was to assess the surface roughness of 2 base metal alloys, submitted to different casting techniques, to determine the influence of surface roughness on loss of mass after polishing compared to commercially pure titanium castings. MATERIAL AND METHODS: Forty disk-shaped wax patterns (8 x 2 mm) were randomly assigned to 5 groups (n=8): (1) CoCr alloy (Wironit), acetylene-oxygen flame casting (WFC), (2) NiCr alloy (Verabond II), acetylene-oxygen flame casting (VFC), (3) CoCr alloy, vacuum casting (WVC), (4) NiCr alloy, vacuum casting (VVC), and (5) commercially pure Ti alloy (Ti) melted by electric arc in argon gas atmosphere. WFC and VFC served as controls. Both NiCr and CoCr alloys were invested in phosphate-bonded investment (Termocast). Ti was invested in ammonium dihydrogen phosphate (Rematitam Plus). The wax patterns and casting techniques were standardized. All specimens were cleaned with airborne-particle abrasion (aluminum oxide). Surface roughness (Ra) was assessed using a surface-test analyzer at 3 different sites. To evaluate the loss of mass, the specimens were weighed on a precision balance, then polished with 150-600 grain sandpaper until clinically acceptable (minimum roughness of 0.09 microm). The specimens were then reweighed. A 1-way analysis of variance (ANOVA) and Tukey HSD test (alpha=.05) were performed. RESULTS: Statistical analysis showed that VVC and WVC had significantly (P=.0050; P=.0057) smoother surfaces (2.43 +/- 0.53 and 2.23 +/- 0.49, respectively) than VFC and WFC (2.99 +/- 0.44 and 2.83 +/- 0.61, respectively), but were not significantly different from Ti (2.49 +/- 0.62). The loss of mass (in %) was not significantly different for any group (3.18 +/- 0.72, 3.14 +/- 0.93, 3.36 +/- 1.05%, and 4.14 +/- 1.28% for VVC, WVC, VFC, and WFC, respectively). The mass loss of Ti was 4.32 +/- 1.16. CONCLUSION: Within the limitations of this study, the base metal alloys submitted to vacuum casting showed decreased surface roughness, similar to that of titanium, compared to base metal alloys submitted to acetylene-oxygen flame casting. There were no significant differences in loss of mass after polishing for all tested specimens.

Análise comparativa da profundidade de fratura de dentes restaurados com núcleos metálicos fundidos ou núcleos de fibra de carbono / Comparative analysis of the depth of fracture of teeth restored with cast metal post and core and carbon fiber posts

Os núcleos metálicos fundidos são muito empregados para a restauração de dentes tratados endodonticamente, mas ultimamente tem havido um crescente interesse pelos sistemas de pinos pré-fabricados, como os de fibra de carbono, que é um material com boas propriedades físicas e mecânicas. O objetivo deste trabalho foi fazer uma comparação, através de ensaios de compressão, esses dois sistemas, tendo-se como grupo controle dentes tratados endodonticamente sem pinos. Concluiu-se que o núcleo metálico fundido provocou fraturas mais profundas, dificultando a restauração dos dentes envolvidos, e que os núcleos de fibra de carbono pareceram oferecer maiores possibilidades de reparo clínico

[Analysis of the point-counting and planimetric methods in the quantification of denture biofilm--a methodological validation study]. / Análise dos métodos de contagem de pontos e planímetro na quantificação do biofilme da dentadura--um estudo de validação metodológica.

Two methods of quantification of the biofilm (point-counting and planimetric) were tested and compared with the paper-weighing method and with the Prosthesis Hygiene Index. The internal surfaces of 62 complete dentures were stained and photographed. The slides were projected on a paper sheet. The total area and the area covered with biofilm were contoured using a black pencil. The point-counting method (experimental 1) was carried out on a mesh of equidistant points. For the planimetric method (experimental 2), the areas of interest were measured by means of a digital planimeter. In the paper-weighing method (control 1) the areas of interest were cut and weighted on a precision scale. In the determination of the Prosthesis Hygiene Index (control 2), the accumulation of biofilm was estimated by means of a scoring method. The results revealed an agreement rate of 82% between the paper-weighing method and the point-counting method, and an agreement rate of 95% between the former and the planimetric method, which was confirmed by high coefficients of correlation (0.98 and 0.99, respectively). The comparison with the Prosthesis Hygiene Index resulted in 55% of agreement with the point-counting method and in 37% of agreement with the planimetric method. The experimental methods can be useful in clinical studies involving the evaluation of the performance of denture cleansers.

Análise dos métodos de contagem de pontos e planímetro na quantificaçäo do biofilme da dentadura - um estudo de validaçäo metodológica / Analysis of the point-counting and planimetric methods in the quantification of the biofilm of dentures - a study of methodological validation

Dois métodos de quantificaçäo de biofilme da dentadura (contagem de pontos e planímetro) foram testados e comparados com o método de pesagem de papel e Indice de Higiene de Prótese. Superfícies internas de 62 próteses foram coradas, fotografadas e as áreas total e do biofilme foram projetadas em papel e contornadas com grafite. O método de contagem de pontos (experimental 1) foi aplicado com uma grade de pontos. para o método do planímetro (experimental 2), as áreas foram medidas com um planímetro digital e para o método de pesagem (controle 1) foram recortadas e pesadas em balança de precisäo. No Indice de Higiene de Prótese (controle 2), utilizou-se a atribuiçäo de escores. Os resultados mostraram uma porcentagem de concordância entre os métodos experimentais e controle 1 de 82 por cento (contagem de pontos) e 95 por cento (planímetro), bem como alto grau de correlaçäo (r = 0,98; r = 0,99) entre os valores obtidos. Quando comparados com o controle 2, houve concordância em 55 por cento (contagem de pontos) e 37 por cento (Planímetro) dos casos. Os métodos experimentais podem ser úteis em estudos clínicos para avaliaçäo da eficácia de agentes de higienizaçäo

Efeito da diluiçäo do Rematitan Plus líquido sobre as dimensöes de uma incrustaçäo MOD / Effects of the dilution of the Rematitan Plus liquid on the dimensions of an inlay MOD

Avaliou-se o efeito da diluiçäo do Rematitan Plus líquido sobre as dimensöes de uma incrustaçäo MOD. A partir de uma matriz, foram confeccionados doze padröes de cera divididos em três grupos. Cada grupo foi incluído em uma diluiçäo de Rematitan Plus líquido dentre aquelas recomendadas pelo fabricante (mínima, máxima e uma intermediária). A partir de marcas de referência feitas na matriz, as peças foram medidas no sentido M-D e V-L. Observou-se que a variaçäo na diluiçäo näo alterava signicativamente as dimensöes da incrustaçäo na maioria dos casos. Além disso, observou-se que as propriedades da cera utilizada como padräo para a confecçäo das incrustaçöes foi mais significativa na alteraçäo dimensional que a diluiçäo do líquido do revestimento